Segmental long bone regeneration guided by degradable synthetic polymeric scaffolds
Xiaowen Xu, Jie Song*()


Figure 4.. BMP-2 delivery from GFOGER-functionalized gels improves bone regeneration compared to collagen foams. (A) 3D μCT reconstructions of radii (left) and mineral density sagittal sections (right). Scale bar: 1 mm. (B) μCT measures of bone volume in radial defects. (C) Bridging score at 8 weeks post-implantation (n = 13). (D) Maximum torque values for 8 weeks radial samples subjected to torsion mechanical testing to failure (n = 5-9). (E) Sections of 8 weeks radial samples stained with Safranin-O/Fast Green. Scale bar: 200 μm. (F) Retention of infrared dye-labelled BMP-2 at implanted defect sites in vivo (n = 6). (G) Quantification of CD45-/CD90+ osteoprogenitor cells present in the defects 7 days post-implantation (n = 4-6). *P < 0.05, ***P < 0.001, ****P < 0.0001, vs. collagen foam/low dose BMP-2. Reproduced from Shekaran et al.126 with permission from Elsevier. 3D: three-dimensional; BMP-2: bone morphogenetic protein 2; GFOGER: α2β1 integrin-specific hexapeptide sequence Gly-Phe-Hyp-Gly-Glu-Ar; μCT: micro-computed tomography.