Three-dimensional biofabrication of an aragonite-enriched self-hardening bone graft substitute and assessment of its osteogenicity in vitro and in vivo
Yunsong Shi1,2, Ruijun He1, Xiangyu Deng1, Zengwu Shao1, Davide Deganello3, Chunze Yan4,*(
), Zhidao Xia2,*()
), Zhidao Xia2,*()
.
Figure 2.. Experimental design of in vitro and in vivo tests. The blocks of HA/aragonite were sterilised by autoclaving at 121°C, 15 psi (1 psi = 6894.76 Pa) for 30 minutes. Further in vitro and in vivo tests were designed to compare between HA/aragonite and clinically-applied gelatine sponge. HA: hydroxyapatite; U & Pb: uranyl acetate and lead citrate staining.
