Hyaluronic acid-based hydrogels with tobacco mosaic virus containing cell adhesive peptide induce bone repair in normal and osteoporotic rats
Jishan Yuan1, Panita Maturavongsadit2,3, Zhihui Zhou1, Bin Lv1, Yuan Lin4, Jia Yang2, Jittima Amie Luckanagul5,6,*()

.

Figure 5.. Histopathological analysis of bone substitute hydrogel implants stained with Masson’s trichrome. (A) Photomicrographs of corresponding Masson’s trichrome-stained sections from each type of hydrogel (original magnification, 40×). In general, Masson’s trichrome stains mature bone with osteoid formation red, whilst blue stain indicates developing calcified bone. (B) Histological scoring of the three types of hydrogels in tissue sections show the different amounts of mature bone area. Data are expressed as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, vs. sham group (one-way analysis of variance). (C) Micro CT images of the cranial-defected bones dissected from non-OVX groups with different types of hydrogel implants at 10 weeks post-surgery. MeHA: methacrylated hyaluronic acid; OVX: ovariectomised; RGD: arginyl-glycyl-aspartic acid; TMV: tobacco mosaic virus.