Jishan Yuan 1, Panita Maturavongsadit 2,3, Zhihui Zhou 1, Bin Lv 1, Yuan Lin 4, Jia Yang 2, Jittima Amie Luckanagul 5,6,*( )
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Figure 5.. Histopathological analysis of bone substitute hydrogel implants stained with Masson’s trichrome. (A) Photomicrographs of corresponding Masson’s trichrome-stained sections from each type of hydrogel (original magnification, 40×). In general, Masson’s trichrome stains mature bone with osteoid formation red, whilst blue stain indicates developing calcified bone. (B) Histological scoring of the three types of hydrogels in tissue sections show the different amounts of mature bone area. Data are expressed as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, vs. sham group (one-way analysis of variance). (C) Micro CT images of the cranial-defected bones dissected from non-OVX groups with different types of hydrogel implants at 10 weeks post-surgery. MeHA: methacrylated hyaluronic acid; OVX: ovariectomised; RGD: arginyl-glycyl-aspartic acid; TMV: tobacco mosaic virus.
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