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Plant-produced recombinant SARS-CoV-2 receptor-binding domain; an economical, scalable biomaterial source for COVID-19 diagnosis
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Figure 4.. Western blot analysis of purified SARS-CoV-2 RBD protein from Nicotiana benthamiana agroinfiltrated with pBY2e-SARS-CoV-2-RBD. (A, B) The purified SARS-CoV-2 RBD protein was loaded at 4 μg/lane under reducing conditions and visualized with InstantBlue? (A) The purified SARS-CoV-2 RBD protein was loaded at 200 ng/lane under reducing conditions and detected with a horseradish peroxidase-conjugated rabbit anti-His antibody (B). M represents the protein molecular weight ladder, and lane 1 shows the purified SARS-CoV-2 RBD. pBY2e: geminiviral vector; RBD: receptor-binding domain; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2. |
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