.

Figure 2.. VACV structural and physical properties. (A) Schematic illustration of the VACV structure, genomic capacity, shape, average size, and surface charge. (B) Fluorescence imaging of green fluorescence protein-tagged VACV (green). (C) Zeta potentials of VACV over the pH 8 and pH 4.5 in 1 mM Tris buffer. Data are expressed as mean ± SD. (D) Atomic force microscopy image of VACV deposited on PDDA-glass substrate. The black arrows point to VACV particles. (E) Representative transmission electron microscopic images of VACV. The white arrows point to (1) outer membrane, (2) core membrane, and (3) surface tubules. Scale bars: 100 μm in B, 20 μm in enlarged part in B, 1 μm in D and 100 nm in E. PDDA: poly(diallyl dimethylammonium chloride); VACV: vaccinia virus. The duplicate samples were measured and two experiments were repeated to acquire the data.