Preclinical evaluation of acute systemic toxicity of magnesium incorporated poly(lactic-co-glycolic acid) porous scaffolds by three-dimensional printing
Jing Long1, Bin Teng2, Wei Zhang1, Long Li1,3, Ming Zhang3, Yingqi Chen3, Zhenyu Yao1, Xiangbo Meng1, Xinluan Wang1, Ling Qin1,4, Yuxiao Lai1,2,5,*()


Figure 2.. In vitro immersion degradation behaviour of scaffolds. (A) Changes in the pH value of different solutions used to soak the PT15M scaffold at 37°C for 24, 48, and 72 hours. (B, C) The concentrations of Mg ions (B) and Ca ions (C) released into serum-free MEM after incubation with PT15M at 37°C for 24, 48, and 72 hours. In the test results, MEM has a higher baseline as it contains calcium chloride. (D) The pH value, Mg, and Ca ion concentrations of MEM incubated with PT, PT5M, PT10M, and PT15M scaffolds at 37°C for 72 hours. Data are expressed as the mean ± SD. (n = 3 and experiments were repeated by twice). Ca: calcium; MEM: serum-free minimum essential medium; Mg: magnesium; PT: poly(lactic-co-glycolic acid)/beta-tricalcium phosphate porous composite scaffolds; PT5M: poly(lactic-co-glycolic acid)/beta-tricalcium phosphate/5 wt% Mg porous composite scaffolds; PT10M: poly(lactic-co-glycolic acid)/beta-tricalcium phosphate/10 wt% Mg porous composite scaffolds; PT15M: poly(lactic-co-glycolic acid)/beta-tricalcium phosphate/15 wt% Mg porous composite scaffolds.