Preclinical evaluation of acute systemic toxicity of magnesium incorporated poly(lactic-co-glycolic acid) porous scaffolds by three-dimensional printing
Jing Long1, Bin Teng2, Wei Zhang1, Long Li1,3, Ming Zhang3, Yingqi Chen3, Zhenyu Yao1, Xiangbo Meng1, Xinluan Wang1, Ling Qin1,4, Yuxiao Lai1,2,5,*()

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Additional Figure 1.. Haematoxylin and eosin staining of heart, liver, lung, spleen, and kidney 1 hour after intraperitoneal injection of extract MEM. The extract MEM was incubated with scaffolds at 37°C for 72 hours. The results showed that the wavy appearance of the myocardium was only apparent in PT15M/pH7/D extract-treated dead mice, while the myocardium in the MEM, PT5M/A, PT10M/A, and PT15M/pH7/A groups remained normal. There were no abnormalities in the liver, lung, spleen, or kidney in any groups. Black arrows indicate loosened cardiac tissues. Scale bars: 200 μm. “A” stands for alive mice, and “D” means dead mice. MEM: serum-free minimum essential medium; Mg: magnesium; PT5M: poly(lactic-co-glycolic acid)/beta-tricalcium phosphate/5 wt% Mg porous composite scaffolds; PT10M: poly(lactic-co-glycolic acid)/beta-tricalcium phosphate/10 wt% Mg porous composite scaffolds; PT15M: poly(lactic-co-glycolic acid)/beta-tricalcium phosphate/15 wt% Mg porous composite scaffolds.