Adipose-derived cells: building blocks of three-dimensional microphysiological systems
Trivia P. Frazier, Katie Hamel, Xiying Wu, Emma Rogers, Haley Lassiter, Jordan Robinson, Omair Mohiuddin, Michael Henderson, Jeffrey M. Gimble
Table 1 Features of adipose-derived stromal/stem cells & stromal vascular cells.
Adipose-derived stromal/stem cells Stromal vascular cells
Shared features · Isolated by enzyme digestion and centrifugation
· Multilineage differentiation potential (adipogenic, chondrogenic, osteogenic)
Distinguishing features Culture adherent & expanded Not exposed to plastic or expanded
Depleted of Hematopoietic Lineages (CD45-) Contains endothelial progenitors, fibroblasts, pericytes, lymphoid and myeloid cells (CD45+)
Higher colony forming unit - fibroblast frequency (> 5%) Lower colony forming unit - fibroblast frequency (1%)