|
|
|
Adipose-derived cells: building blocks of three-dimensional microphysiological systems
|
|
Trivia P. Frazier, Katie Hamel, Xiying Wu, Emma Rogers, Haley Lassiter, Jordan Robinson, Omair Mohiuddin, Michael Henderson, Jeffrey M. Gimble
|
|
| |
Table 1 Features of adipose-derived stromal/stem cells & stromal vascular cells.
|
|
| Adipose-derived stromal/stem cells | Stromal vascular cells | | Shared features | · Isolated by enzyme digestion and centrifugation | | · Multilineage differentiation potential (adipogenic, chondrogenic, osteogenic) | | Distinguishing features | Culture adherent & expanded | Not exposed to plastic or expanded | | Depleted of Hematopoietic Lineages (CD45-) | Contains endothelial progenitors, fibroblasts, pericytes, lymphoid and myeloid cells (CD45+) | | Higher colony forming unit - fibroblast frequency (> 5%) | Lower colony forming unit - fibroblast frequency (1%) |
|
|
|