Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts

Yang Zhao, Qing Sun, Bo Huo

Additional Figure 6. Fluorescence intensity of osteogenic differentiation markers in MSCs cultured on micropatterned substrates. (A-F) Statistical results of fluorescent intensity of ALP, COL I, and OCN, respectively, after culture for 3 days (A, C, E) or 7 days (B, D, F). Data are presented as mean ± SD (n = 3). *P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc analysis). ALP: alkaline phosphatase; COL I: type I collagen; LL: large circles with large spacing; MSC: mesenchymal stem cell; OCN: osteocalcin; SL: small circles with large spacing; SS: small circles with small spacing.