Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts

Yang Zhao, Qing Sun, Bo Huo

Additional Figure 7. Fluorescence intensity of osteogenic differentiation markers in MC3T3-E1 cells cultured on micropatterned substrates. (A-F) Statistical results of fluorescent intensity of ALP, COL I, and OCN in cells cultured for 3 days (A, C, E) or 7 days (B, D, F), respectively. Data are presented as mean ± SD (n = 3). *P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc analysis). ALP: alkaline phosphatase; COL I: type I collagen; LL: large circles with large spacing; OCN: osteocalcin; SL: small circles with large spacing; SS: small circles with small spacing.