Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts

Yang Zhao, Qing Sun, Bo Huo

Additional Figure 10. Cell phenotypes and area after CD treatment for 24 hours. (A) Bright-field images of the cell on the micropatterned substrates. Cells were shrunk obviously. Scale bars: 50 μm. (B) Statistical analysis of the spreading area of the cells. Data are presented as mean ± SD (n = 3). *P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc analysis). CD: cytochalasin D; LL: large circles with large spacing; MSC: mesenchymal stem cell; SL: small circles with large spacing; SS: small circles with small spacing.