Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts

Yang Zhao, Qing Sun, Bo Huo

Additional Figure 14. Statistical results of YAP localisation after CD treatment. (A) Fluorescent images of YAP. Nuclear transfer of YAP could not be observed. The yellow circles represent the location of micropatterned islands. Scale bars: 50 μm. (B) Statistical analyses of the nuclear/cytoplasmic ratio of YAP in MSCs and MC3T3-E1 cells. Data are presented as mean ± SD (n = 3), and were analysed by one-way analysis of variance followed by Tukey’s post hoc analysis. CD: cytochalasin D; LL: large circles with large spacing; MSC: mesenchymal stem cell; SL: small circles with large spacing; SS: small circles with small spacing; YAP: yes-associated proteins.