Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts

Yang Zhao, Qing Sun, Bo Huo

Figure 3. P-MLC2 in cells cultured on micropatterned substrates. (A, B) Fluorescent images of individual MSCs (A) and MC3T3-E1 cells (B) stained with TRITC-labeled P-MLC2 (red) with Hoechst 33342-stained nuclei (blue) at 3 days after seeding. The yellow circles represent the location of micropatterned islands. The gray shape represents the spreading shape of cells. Scale bars: 50 μm. (C, D) Statistical results of P-MLC2 in MSCs (C) and MC3T3-E1 cells (D). Data are presented as mean ± SD (n = 3). *P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc analysis). CON: control (freely spreading group); LL: large circles with large spacing; MSC: mesenchymal stem cell; P-MLC2: phosphorylated myosin light chain 2; PTN: pattern; SL: small circles with large spacing; SS: small circles with small spacing; TRITC: tetraethyl rhodamine isothiocyanate.