Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts

Yang Zhao, Qing Sun, Bo Huo

Figure 4. FITC-labeled fluorescent images of the osteogenic differentiation markers ALP, COL I, and OCN in MSCs and MC3T3-E1 cells cultured on a micropatterned substrate after 3 (ALP) or 7 (COL I, OCN) days of culture. Cells might spread out into triangles, dumbbells, etc. The yellow circles indicate the location of micropatterned islands. The gray shape represents the spreading shape of cells. Scale bars: 50 μm. ALP: alkaline phosphatase; COL I: type I collagen; FITC: fluorescein isothiocyanate; MSC: mesenchymal stem cell; OCN: osteocalcin.