Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts

Yang Zhao, Qing Sun, Bo Huo

Figure 6. YAP staining in cells cultured on a micropatterned substrate. (A) Fluorescent images of individual cells stained with fluorescein isothiocyanate-labeled YAP (green) with Hoechst 33342-stained nuclei (blue) after 3 or 7 days of culture on the micropatterned substrate. Yellow circles indicate the location of micropatterned islands, and yellow dotted circles show the outlines of nuclei. The gray shape represents the spreading shape of cells. Scale bars: 50 μm. (B, C) Statistical analyses of the fluorescence intensity of YAP in MSCs and MC3T3-E1 cells, respectively. (D, E) Statistical analyses of the nuclear/cytoplasmic ratio of YAP in MSCs and MC3T3-E1 cells, respectively. Data are presented as mean ± SD (n = 3). *P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc analysis). CON: control (free spreading group); LL: large circles with large spacing; MSC: mesenchymal stem cell; PTN: pattern; SL: small circles with large spacing; SS: small circles with small spacing; YAP: yes-associated proteins.