1. Fioretta, E. S.; Motta, S. E.; Lintas, V.; Loerakker, S.; Parker, K. K.; Baaijens, F. P. T.; Falk, V.; Hoerstrup, S. P.; Emmert, M. Y. Next-generation tissue-engineered heart valves with repair, remodelling and regeneration capacity. Nat Rev Cardiol. 2021, 18, 92-116.  
2. Eoh, J. H.; Shen, N.; Burke, J. A.; Hinderer, S.; Xia, Z.; Schenke-Layland, K.; Gerecht, S. Enhanced elastin synthesis and maturation in human vascular smooth muscle tissue derived from induced-pluripotent stem cells. Acta Biomater. 2017, 52, 49-59.  
3. Henaine, R.; Roubertie, F.; Vergnat, M.; Ninet, J. Valve replacement in children: a challenge for a whole life. Arch Cardiovasc Dis. 2012, 105, 517-528.  
4. Kaneko, T.; Cohn, L. H.; Aranki, S. F. Tissue valve is the preferred option for patients aged 60 and older. Circulation. 2013, 128, 1365-1371.  
5. Emmert, M. Y.; Hoerstrup, S. P. Challenges in translating tissue engineered heart valves into clinical practice. Eur Heart J. 2017, 38, 619-621.  
6. Zhang, B. L.; Bianco, R. W.; Schoen, F. J. Preclinical assessment of cardiac valve substitutes: current status and considerations for engineered tissue heart valves. Front Cardiovasc Med. 2019, 6, 72.  
7. Chester, A. H.; Grande-Allen, K. J. Which biological properties of heart valves are relevant to tissue engineering? Front Cardiovasc Med. 2020, 7, 63.  
8. Quint, C.; Kondo, Y.; Manson, R. J.; Lawson, J. H.; Dardik, A.; Niklason, L. E. Decellularized tissue-engineered blood vessel as an arterial conduit. Proc Natl Acad Sci U S A. 2011, 108, 9214-9219.  
9. Dahl, S. L.; Kypson, A. P.; Lawson, J. H.; Blum, J. L.; Strader, J. T.; Li, Y.; Manson, R. J.; Tente, W. E.; DiBernardo, L.; Hensley, M. T.; Carter, R.; Williams, T. P.; Prichard, H. L.; Dey, M. S.; Begelman, K. G.; Niklason, L. E. Readily available tissue-engineered vascular grafts. Sci Transl Med. 2011, 3, 68ra69.  
10. Majid, Q. A.; Fricker, A. T. R.; Gregory, D. A.; Davidenko, N.; Hernandez Cruz, O.; Jabbour, R. J.; Owen, T. J.; Basnett, P.; Lukasiewicz, B.; Stevens, M.; Best, S.; Cameron, R.; Sinha, S.; Harding, S. E.; Roy, I. Natural biomaterials for cardiac tissue engineering: a highly biocompatible solution. Front Cardiovasc Med. 2020, 7, 554597.  
11. Xue, Y.; Sant, V.; Phillippi, J.; Sant, S. Biodegradable and biomimetic elastomeric scaffolds for tissue-engineered heart valves. Acta Biomater. 2017, 48, 2-19.  
12. Mirani, B.; Parvin Nejad, S.; Simmons, C. A. Recent progress toward clinical translation of tissue-engineered heart valves. Can J Cardiol. 2021, 37, 1064-1077.  
13. Hayashida, K.; Kanda, K.; Yaku, H.; Ando, J.; Nakayama, Y. Development of an in vivo tissue-engineered, autologous heart valve (the biovalve): preparation of a prototype model. J Thorac Cardiovasc Surg. 2007, 134, 152-159.  
14. Yamanami, M.; Yahata, Y.; Uechi, M.; Fujiwara, M.; Ishibashi-Ueda, H.; Kanda, K.; Watanabe, T.; Tajikawa, T.; Ohba, K.; Yaku, H.; Nakayama, Y. Development of a completely autologous valved conduit with the sinus of Valsalva using in-body tissue architecture technology: a pilot study in pulmonary valve replacement in a beagle model. Circulation. 2010, 122, S100-106.  
15. Motta, S. E.; Lintas, V.; Fioretta, E. S.; Dijkman, P. E.; Putti, M.; Caliskan, E.; Rodriguez Cetina Biefer, H.; Lipiski, M.; Sauer, M.; Cesarovic, N.; Hoerstrup, S. P.; Emmert, M. Y. Human cell-derived tissue-engineered heart valve with integrated Valsalva sinuses: towards native-like transcatheter pulmonary valve replacements. NPJ Regen Med. 2019, 4, 14.  
16. Schmitt, B.; Spriestersbach, H.; D, O. H. I.; Radtke, T.; Bartosch, M.; Peters, H.; Sigler, M.; Frese, L.; Dijkman, P. E.; Baaijens, F. P.; Hoerstrup, S. P.; Berger, F. Percutaneous pulmonary valve replacement using completely tissue-engineered off-the-shelf heart valves: six-month in vivo functionality and matrix remodelling in sheep. EuroIntervention. 2016, 12, 62-70.  
17. Capulli, A. K.; Emmert, M. Y.; Pasqualini, F. S.; Kehl, D.; Caliskan, E.; Lind, J. U.; Sheehy, S. P.; Park, S. J.; Ahn, S.; Weber, B.; Goss, J. A.; Hoerstrup, S. P.; Parker, K. K. JetValve: Rapid manufacturing of biohybrid scaffolds for biomimetic heart valve replacement. Biomaterials. 2017, 133, 229-241.  
18. Guo, G.; Jin, L.; Wu, B.; He, H.; Yang, F.; Xu, L.; Lei, Y.; Wang, Y. A method for simultaneously crosslinking and functionalizing extracellular matrix-based biomaterials as bioprosthetic heart valves with enhanced endothelialization and reduced inflammation. Acta Biomater. 2021, 119, 89-100.  
19. Lovekamp, J. J.; Simionescu, D. T.; Mercuri, J. J.; Zubiate, B.; Sacks, M. S.; Vyavahare, N. R. Stability and function of glycosaminoglycans in porcine bioprosthetic heart valves. Biomaterials. 2006, 27, 1507-1518.  
20. Jana, S.; Franchi, F.; Lerman, A. Trilayered tissue structure with leaflet-like orientations developed through in vivo tissue engineering. Biomed Mater. 2019, 15, 015004.  
21. Soares, A. L.; Oomens, C. W.; Baaijens, F. P. A computational model to describe the collagen orientation in statically cultured engineered tissues. Comput Methods Biomech Biomed Engin. 2014, 17, 251-262.  
22. Emmert, M. Y.; Schmitt, B. A.; Loerakker, S.; Sanders, B.; Spriestersbach, H.; Fioretta, E. S.; Bruder, L.; Brakmann, K.; Motta, S. E.; Lintas, V.; Dijkman, P. E.; Frese, L.; Berger, F.; Baaijens, F. P. T.; Hoerstrup, S. P. Computational modeling guides tissue-engineered heart valve design for long-term in vivo performance in a translational sheep model. Sci Transl Med. 2018, 10, eaan4587.  
23. Flanagan, T. C.; Sachweh, J. S.; Frese, J.; Schnöring, H.; Gronloh, N.; Koch, S.; Tolba, R. H.; Schmitz-Rode, T.; Jockenhoevel, S. In vivo remodeling and structural characterization of fibrin-based tissue-engineered heart valves in the adult sheep model. Tissue Eng Part A. 2009, 15, 2965-2976.  
24. Wu, H.; Xu, Z. W.; Liu, X. M.; Gong, D.; Wan, J. Y.; Xu, X. F.; Zhou, Z. F.; Li, W. B. An in vivo model of in situ implantation using pulmonary valved conduit in large animals under off-pump condition. Chin Med J (Engl). 2013, 126, 4540-4544.  
25. Zakko, J.; Blum, K. M.; Drews, J. D.; Wu, Y. L.; Hatoum, H.; Russell, M.; Gooden, S.; Heitkemper, M.; Conroy, O.; Kelly, J.; Carey, S.; Sacks, M.; Texter, K.; Ragsdale, E.; Strainic, J.; Bocks, M.; Wang, Y.; Dasi, L. P.; Armstrong, A. K.; Breuer, C. Development of tissue engineered heart valves for percutaneous transcatheter delivery in a fetal ovine model. JACC Basic Transl Sci. 2020, 5, 815-828.  
26. Coyan, G. N.; D’Amore, A.; Matsumura, Y.; Pedersen, D. D.; Luketich, S. K.; Shanov, V.; Katz, W. E.; David, T. E.; Wagner, W. R.; Badhwar, V. In vivo functional assessment of a novel degradable metal and elastomeric scaffold-based tissue engineered heart valve. J Thorac Cardiovasc Surg. 2019, 157, 1809-1816.  
27. Schlegel, F.; Salameh, A.; Oelmann, K.; Halling, M.; Dhein, S.; Mohr, F. W.; Dohmen, P. M. Injectable tissue engineered pulmonary heart valve implantation into the pig model: A feasibility study. Med Sci Monit Basic Res. 2015, 21, 135-140.  
28. Bianco, R. W.; St Cyr, J. A.; Schneider, J. R.; Rasmussen, T. M.; Clack, R. M.; Shim, H. S.; Sandstad, J.; Rysavy, J.; Foker, J. E. Canine model for long-term evaluation of prosthetic mitral valves. J Surg Res. 1986, 41, 134-140.  
29. Ye, X.; Bhushan, B.; Zhou, M.; Lei, W. The surface microstructure of cusps and leaflets in rabbit and mouse heart valves. Beilstein J Nanotechnol. 2014, 5, 622-629.  
30. Weber, B.; Dijkman, P. E.; Scherman, J.; Sanders, B.; Emmert, M. Y.; Grünenfelder, J.; Verbeek, R.; Bracher, M.; Black, M.; Franz, T.; Kortsmit, J.; Modregger, P.; Peter, S.; Stampanoni, M.; Robert, J.; Kehl, D.; van Doeselaar, M.; Schweiger, M.; Brokopp, C. E.; Wälchli, T.; Falk, V.; Zilla, P.; Driessen-Mol, A.; Baaijens, F. P.; Hoerstrup, S. P. Off-the-shelf human decellularized tissue-engineered heart valves in a non-human primate model. Biomaterials. 2013, 34, 7269-7280.  
31. Smith, M. R.; Wood, W. B., Jr. An experimental analysis of the curative action of penicillin in acute bacterial infections. III. The effect of suppuration upon the antibacterial action of the drug. J Exp Med. 1956, 103, 509-522.  
32. Kim, M. S.; Jeong, S.; Lim, H. G.; Kim, Y. J. Differences in xenoreactive immune response and patterns of calcification of porcine and bovine tissues in α-Gal knock-out and wild-type mouse implantation models. Eur J Cardiothorac Surg. 2015, 48, 392-399.  
33. Christ, T.; Dohmen, P. M.; Holinski, S.; Schönau, M.; Heinze, G.; Konertz, W. Suitability of the rat subdermal model for tissue engineering of heart valves. Med Sci Monit Basic Res. 2014, 20, 194-199.  
34. Assmann, A.; Delfs, C.; Munakata, H.; Schiffer, F.; Horstkötter, K.; Huynh, K.; Barth, M.; Stoldt, V. R.; Kamiya, H.; Boeken, U.; Lichtenberg, A.; Akhyari, P. Acceleration of autologous in vivo recellularization of decellularized aortic conduits by fibronectin surface coating. Biomaterials. 2013, 34, 6015-6026.  
35. Rashid, S. T.; Salacinski, H. J.; Hamilton, G.; Seifalian, A. M. The use of animal models in developing the discipline of cardiovascular tissue engineering: a review. Biomaterials. 2004, 25, 1627-1637.  
36. Driessen-Mol, A.; Emmert, M. Y.; Dijkman, P. E.; Frese, L.; Sanders, B.; Weber, B.; Cesarovic, N.; Sidler, M.; Leenders, J.; Jenni, R.; Grünenfelder, J.; Falk, V.; Baaijens, F. P. T.; Hoerstrup, S. P. Transcatheter implantation of homologous “off-the-shelf” tissue-engineered heart valves with self-repair capacity: long-term functionality and rapid in vivo remodeling in sheep. J Am Coll Cardiol. 2014, 63, 1320-1329.  
37. Shinoka, T.; Ma, P. X.; Shum-Tim, D.; Breuer, C. K.; Cusick, R. A.; Zund, G.; Langer, R.; Vacanti, J. P.; Mayer, J. E., Jr. Tissue-engineered heart valves. Autologous valve leaflet replacement study in a lamb model. Circulation. 1996, 94, II164-168.  
38. Schroeder, F.; Polzer, S.; Slažanský, M.; Man, V.; Skácel, P. Predictive capabilities of various constitutive models for arterial tissue. J Mech Behav Biomed Mater. 2018, 78, 369-380.  
39. Swindle, M. M.; Makin, A.; Herron, A. J.; Clubb, F. J., Jr.; Frazier, K. S. Swine as models in biomedical research and toxicology testing. Vet Pathol. 2012, 49, 344-356.  
40. Grehan, J. F.; Hilbert, S. L.; Ferrans, V. J.; Droel, J. S.; Salerno, C. T.; Bianco, R. W. Development and evaluation of a swine model to assess the preclinical safety of mechanical heart valves. J Heart Valve Dis. 2000, 9, 710-719; discussion 719-720.  
41. Canty, J. M., Jr.; Suzuki, G.; Banas, M. D.; Verheyen, F.; Borgers, M.; Fallavollita, J. A. Hibernating myocardium: chronically adapted to ischemia but vulnerable to sudden death. Circ Res. 2004, 94, 1142-1149.  
42. Gallo, M.; Poser, H.; Bottio, T.; Bonetti, A.; Franci, P.; Naso, F.; Buratto, E.; Zanella, F.; Perona, G.; Dal Lin, C.; Bianco, R.; Spina, M.; Busetto, R.; Marchini, M.; Ortolani, F.; Iop, L.; Gerosa, G. The Vietnamese pig as a translational animal model to evaluate tissue engineered heart valves: promising early experience. Int J Artif Organs. 2017, 40, 142-149.  
43. Gallo, M.; Bianco, R.; Bottio, T.; Naso, F.; Franci, P.; Zanella, F.; Perona, G.; Busetto, R.; Spina, M.; Gandaglia, A.; Gerosa, G. Tissue-engineered heart valves: intra-operative protocol. J Cardiovasc Transl Res. 2013, 6, 660-661.  
44. Gallo, M.; Naso, F.; Poser, H.; Rossi, A.; Franci, P.; Bianco, R.; Micciolo, M.; Zanella, F.; Cucchini, U.; Aresu, L.; Buratto, E.; Busetto, R.; Spina, M.; Gandaglia, A.; Gerosa, G. Physiological performance of a detergent decellularized heart valve implanted for 15 months in Vietnamese pigs: surgical procedure, follow-up, and explant inspection. Artif Organs. 2012, 36, E138-150.  
45. Boudjemline, Y.; Agnoletti, G.; Bonnet, D.; Behr, L.; Borenstein, N.; Sidi, D.; Bonhoeffer, P. Steps toward the percutaneous replacement of atrioventricular valves: an experimental study. J Am Coll Cardiol. 2005, 46, 360-365.  
46. Dewey, T. M.; Walther, T.; Doss, M.; Brown, D.; Ryan, W. H.; Svensson, L.; Mihaljevic, T.; Hambrecht, R.; Schuler, G.; Wimmer-Greinecker, G.; Mohr, F. W.; Mack, M. J. Transapical aortic valve implantation: an animal feasibility study. Ann Thorac Surg. 2006, 82, 110-116.  
47. Lutter, G.; Kuklinski, D.; Berg, G.; Von Samson, P.; Martin, J.; Handke, M.; Uhrmeister, P.; Beyersdorf, F. Percutaneous aortic valve replacement: an experimental study. I. Studies on implantation. J Thorac Cardiovasc Surg. 2002, 123, 768-776.  
48. Walther, T.; Dewey, T.; Wimmer-Greinecker, G.; Doss, M.; Hambrecht, R.; Schuler, G.; Mohr, F. W.; Mack, M. Transapical approach for sutureless stent-fixed aortic valve implantation: experimental results. Eur J Cardiothorac Surg. 2006, 29, 703-708.  
49. Kokozidou, M.; Katsargyris, A.; Verhoeven, E. L. G.; Schulze-Tanzil, G. Vascular access animal models used in research. Ann Anat. 2019, 225, 65-75.  
50. Camacho, P.; Fan, H.; Liu, Z.; He, J. Q. Large mammalian animal models of heart disease. J Cardiovasc Dev Dis. 2016, 3, 30.  
51. Iwai, S.; Torikai, K.; Coppin, C. M.; Sawa, Y. Minimally immunogenic decellularized porcine valve provides in situ recellularization as a stentless bioprosthetic valve. J Artif Organs. 2007, 10, 29-35.  
52. Yang, H.; Shao, N.; Holmström, A.; Zhao, X.; Chour, T.; Chen, H.; Itzhaki, I.; Wu, H.; Ameen, M.; Cunningham, N. J.; Tu, C.; Zhao, M. T.; Tarantal, A. F.; Abilez, O. J.; Wu, J. C. Transcriptome analysis of non-human primate-induced pluripotent stem cell-derived cardiomyocytes in 2D monolayer culture vs. 3D engineered heart tissue. Cardiovasc Res. 2021, 117, 2125-2136.  
53. Rahman, M. A.; Robert-Guroff, M. Accelerating HIV vaccine development using non-human primate models. Expert Rev Vaccines. 2019, 18, 61-73.  
54. Brok, H. P.; Bauer, J.; Jonker, M.; Blezer, E.; Amor, S.; Bontrop, R. E.; Laman, J. D.; t Hart, B. A. Non-human primate models of multiple sclerosis. Immunol Rev. 2001, 183, 173-185.  
55. Lu, T.; Yang, B.; Wang, R.; Qin, C. Xenotransplantation: current status in preclinical research. Front Immunol. 2019, 10, 3060.  
56. Anderson, J. M.; Rodriguez, A.; Chang, D. T. Foreign body reaction to biomaterials. Semin Immunol. 2008, 20, 86-100.  
57. Raasch, M.; Rennert, K.; Jahn, T.; Peters, S.; Henkel, T.; Huber, O.; Schulz, I.; Becker, H.; Lorkowski, S.; Funke, H.; Mosig, A. Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication. 2015, 7, 015013.  
58. Rath, S.; Salinas, M.; Villegas, A. G.; Ramaswamy, S. Differentiation and distribution of marrow stem cells in flex-flow environments demonstrate support of the valvular phenotype. PLoS One. 2015, 10, e0141802.  
59. Flanagan, T. C.; Cornelissen, C.; Koch, S.; Tschoeke, B.; Sachweh, J. S.; Schmitz-Rode, T.; Jockenhoevel, S. The in vitro development of autologous fibrin-based tissue-engineered heart valves through optimised dynamic conditioning. Biomaterials. 2007, 28, 3388-3397.  
60. Deb, N.; Ali, M. S.; Mathews, A.; Chang, Y. W.; Lacerda, C. M. Shear type and magnitude affect aortic valve endothelial cell morphology, orientation, and differentiation. Exp Biol Med (Maywood). 2021, 246, 2278-2289.  
61. Li, A.; Tan, L.; Zhang, S.; Tao, J.; Wang, Z.; Wei, D. Low shear stress-induced endothelial mesenchymal transformation via the down-regulation of TET2. Biochem Biophys Res Commun. 2021, 545, 20-26.  
62. Bazan, O.; Simbara, M. M. O.; Ortiz, J. P.; Malmonge, S. M.; Andrade, A.; Yanagihara, J. I. In vitro hydrodynamic evaluation of a scaffold for heart valve tissue engineering. Artif Organs. 2019, 43, 195-198.  
63. Tefft, B. J.; Choe, J. A.; Young, M. D.; Hennessy, R. S.; Morse, D. W.; Bouchard, J. A.; Hedberg, H. J.; Consiglio, J. F.; Dragomir-Daescu, D.; Simari, R. D.; Lerman, A. Cardiac valve bioreactor for physiological conditioning and hydrodynamic performance assessment. Cardiovasc Eng Technol. 2019, 10, 80-94.  
64. Kim, J.; Lee, Y.; Choi, S.; Ha, H. Pulsatile flow pump based on an iterative controlled piston pump actuator as an in-vitro cardiovascular flow model. Med Eng Phys. 2020, 77, 118-124.  
65. Qian, T.; Gil, D. A.; Contreras Guzman, E.; Gastfriend, B. D.; Tweed, K. E.; Palecek, S. P.; Skala, M. C. Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction. Lab Chip. 2020, 20, 3744-3756.  
66. Posmantur, R.; Hayes, R. L.; Dixon, C. E.; Taft, W. C. Neurofilament 68 and neurofilament 200 protein levels decrease after traumatic brain injury. J Neurotrauma. 1994, 11, 533-545.  
67. Hildebrand, D. K.; Wu, Z. J.; Mayer, J. E., Jr.; Sacks, M. S. Design and hydrodynamic evaluation of a novel pulsatile bioreactor for biologically active heart valves. Ann Biomed Eng. 2004, 32, 1039-1049.  
68. Gandaglia, A.; Bagno, A.; Naso, F.; Spina, M.; Gerosa, G. Cells, scaffolds and bioreactors for tissue-engineered heart valves: a journey from basic concepts to contemporary developmental innovations. Eur J Cardiothorac Surg. 2011, 39, 523-531.  
69. Ramaswamy, S.; Boronyak, S. M.; Le, T.; Holmes, A.; Sotiropoulos, F.; Sacks, M. S. A novel bioreactor for mechanobiological studies of engineered heart valve tissue formation under pulmonary arterial physiological flow conditions. J Biomech Eng. 2014, 136, 121009.  
70. Nguemgo Kouam, P.; Bühler, H.; Hero, T.; Adamietz, I. A. The increased adhesion of tumor cells to endothelial cells after irradiation can be reduced by FAK-inhibition. Radiat Oncol. 2019, 14, 25.  
71. Hampel, U.; Garreis, F.; Burgemeister, F.; Eßel, N.; Paulsen, F. Effect of intermittent shear stress on corneal epithelial cells using an in vitro flow culture model. Ocul Surf. 2018, 16, 341-351.  
72. Helle, E.; Ampuja, M.; Antola, L.; Kivelä, R. Flow-induced transcriptomic remodeling of endothelial cells derived from human induced pluripotent stem cells. Front Physiol. 2020, 11, 591450.  
73. Moon du, G.; Christ, G.; Stitzel, J. D.; Atala, A.; Yoo, J. J. Cyclic mechanical preconditioning improves engineered muscle contraction. Tissue Eng Part A. 2008, 14, 473-482.  
74. Sun, L.; Qu, L.; Zhu, R.; Li, H.; Xue, Y.; Liu, X.; Fan, J.; Fan, H. Effects of mechanical stretch on cell proliferation and matrix formation of mesenchymal stem cell and anterior cruciate ligament fibroblast. Stem Cells Int. 2016, 2016, 9842075.  
75. Ku, C. H.; Johnson, P. H.; Batten, P.; Sarathchandra, P.; Chambers, R. C.; Taylor, P. M.; Yacoub, M. H.; Chester, A. H. Collagen synthesis by mesenchymal stem cells and aortic valve interstitial cells in response to mechanical stretch. Cardiovasc Res. 2006, 71, 548-556.  
76. Syedain, Z. H.; Tranquillo, R. T. Controlled cyclic stretch bioreactor for tissue-engineered heart valves. Biomaterials. 2009, 30, 4078-4084.  
77. Engelmayr, G. C., Jr.; Rabkin, E.; Sutherland, F. W.; Schoen, F. J.; Mayer, J. E., Jr.; Sacks, M. S. The independent role of cyclic flexure in the early in vitro development of an engineered heart valve tissue. Biomaterials. 2005, 26, 175-187.  
78. Engelmayr, G. C., Jr.; Hildebrand, D. K.; Sutherland, F. W.; Mayer, J. E., Jr.; Sacks, M. S. A novel bioreactor for the dynamic flexural stimulation of tissue engineered heart valve biomaterials. Biomaterials. 2003, 24, 2523-2532.  
79. Kutikhin, A. G.; Sinitsky, M. Y.; Yuzhalin, A. E.; Velikanova, E. A. Shear stress: an essential driver of endothelial progenitor cells. J Mol Cell Cardiol. 2018, 118, 46-69.  
80. Yang, Z.; Xia, W. H.; Zhang, Y. Y.; Xu, S. Y.; Liu, X.; Zhang, X. Y.; Yu, B. B.; Qiu, Y. X.; Tao, J. Shear stress-induced activation of Tie2-dependent signaling pathway enhances reendothelialization capacity of early endothelial progenitor cells. J Mol Cell Cardiol. 2012, 52, 1155-1163.  
81. Campinho, P.; Vilfan, A.; Vermot, J. Blood flow forces in shaping the vascular system: a focus on endothelial cell behavior. Front Physiol. 2020, 11, 552.  
82. da Silva, R. A.; Fernandes, C.; Feltran, G. D. S.; Gomes, A. M.; de Camargo Andrade, A. F.; Andia, D. C.; Peppelenbosch, M. P.; Zambuzzi, W. F. Laminar shear stress-provoked cytoskeletal changes are mediated by epigenetic reprogramming of TIMP1 in human primary smooth muscle cells. J Cell Physiol. 2019, 234, 6382-6396.  
83. Gonzalez, B. A.; Perez-Nevarez, M.; Mirza, A.; Perez, M. G.; Lin, Y. M.; Hsu, C. D.; Caobi, A.; Raymond, A.; Gomez Hernandez, M. E.; Fernandez-Lima, F.; George, F.; Ramaswamy, S. Physiologically relevant fluid-induced oscillatory shear stress stimulation of mesenchymal stem cells enhances the engineered valve matrix phenotype. Front Cardiovasc Med. 2020, 7, 69.  
84. Li, J.; He, Y.; Bu, H.; Wang, M.; Yu, J.; Li, L.; Li, H.; Zhang, X.; Cui, X.; Cheng, M. Oscillating shear stress mediates mesenchymal transdifferentiation of EPCs by the Kir2.1 channel. Heart Vessels. 2020, 35, 1473-1482.  
85. Gao, Y.; Cui, X.; Wang, M.; Zhang, Y.; He, Y.; Li, L.; Li, H.; Zhang, X.; Cheng, M. Oscillatory shear stress induces the transition of EPCs into mesenchymal cells through ROS/PKCζ/p53 pathway. Life Sci. 2020, 253, 117728.  
86. Converse, G. L.; Buse, E. E.; Neill, K. R.; McFall, C. R.; Lewis, H. N.; VeDepo, M. C.; Quinn, R. W.; Hopkins, R. A. Design and efficacy of a single-use bioreactor for heart valve tissue engineering. J Biomed Mater Res B Appl Biomater. 2017, 105, 249-259.  
87. Jockenhoevel, S.; Zund, G.; Hoerstrup, S. P.; Schnell, A.; Turina, M. Cardiovascular tissue engineering: a new laminar flow chamber for in vitro improvement of mechanical tissue properties. ASAIO J. 2002, 48, 8-11.  
88. Engelmayr, G. C., Jr.; Sales, V. L.; Mayer, J. E., Jr.; Sacks, M. S. Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Biomaterials. 2006, 27, 6083-6095.  
89. Ramaswamy, S.; Gottlieb, D.; Engelmayr, G. C., Jr.; Aikawa, E.; Schmidt, D. E.; Gaitan-Leon, D. M.; Sales, V. L.; Mayer, J. E., Jr.; Sacks, M. S. The role of organ level conditioning on the promotion of engineered heart valve tissue development in vitro using mesenchymal stem cells. Biomaterials. 2010, 31, 1114-1125.  
90. Mongkoldhumrongkul, N.; Latif, N.; Yacoub, M. H.; Chester, A. H. Effect of side-specific valvular shear stress on the content of extracellular matrix in aortic valves. Cardiovasc Eng Technol. 2018, 9, 151-157.  
91. Engelmayr, G. C., Jr.; Soletti, L.; Vigmostad, S. C.; Budilarto, S. G.; Federspiel, W. J.; Chandran, K. B.; Vorp, D. A.; Sacks, M. S. A novel flex-stretch-flow bioreactor for the study of engineered heart valve tissue mechanobiology. Ann Biomed Eng. 2008, 36, 700-712.  
92. Vozzi, F.; Bianchi, F.; Ahluwalia, A.; Domenici, C. Hydrostatic pressure and shear stress affect endothelin-1 and nitric oxide release by endothelial cells in bioreactors. Biotechnol J. 2014, 9, 146-154.  
93. VeDepo, M. C.; Buse, E. E.; Paul, A.; Converse, G. L.; Hopkins, R. A. Non-physiologic bioreactor processing conditions for heart valve tissue engineering. Cardiovasc Eng Technol. 2019, 10, 628-637.  
94. Hutmacher, D. W.; Singh, H. Computational fluid dynamics for improved bioreactor design and 3D culture. Trends Biotechnol. 2008, 26, 166-172.  
95. Williams, A.; Nasim, S.; Salinas, M.; Moshkforoush, A.; Tsoukias, N.; Ramaswamy, S. A “sweet-spot” for fluid-induced oscillations in the conditioning of stem cell-based engineered heart valve tissues. J Biomech. 2017, 65, 40-48.  
96. Salinas, M.; Ramaswamy, S. Computational simulations predict a key role for oscillatory fluid shear stress in de novo valvular tissue formation. J Biomech. 2014, 47, 3517-3523.  
97. Lichtenberg, A.; Tudorache, I.; Cebotari, S.; Suprunov, M.; Tudorache, G.; Goerler, H.; Park, J. K.; Hilfiker-Kleiner, D.; Ringes-Lichtenberg, S.; Karck, M.; Brandes, G.; Hilfiker, A.; Haverich, A. Preclinical testing of tissue-engineered heart valves re-endothelialized under simulated physiological conditions. Circulation. 2006, 114, I559-565.  
98. Lichtenberg, A.; Tudorache, I.; Cebotari, S.; Ringes-Lichtenberg, S.; Sturz, G.; Hoeffler, K.; Hurscheler, C.; Brandes, G.; Hilfiker, A.; Haverich, A. In vitro re-endothelialization of detergent decellularized heart valves under simulated physiological dynamic conditions. Biomaterials. 2006, 27, 4221-4229.  
99. Santoro, R.; Venkateswaran, S.; Amadeo, F.; Zhang, R.; Brioschi, M.; Callanan, A.; Agrifoglio, M.; Banfi, C.; Bradley, M.; Pesce, M. Acrylate-based materials for heart valve scaffold engineering. Biomater Sci. 2017, 6, 154-167.  
100. Best, C. A.; Szafron, J. M.; Rocco, K. A.; Zbinden, J.; Dean, E. W.; Maxfield, M. W.; Kurobe, H.; Tara, S.; Bagi, P. S.; Udelsman, B. V.; Khosravi, R.; Yi, T.; Shinoka, T.; Humphrey, J. D.; Breuer, C. K. Differential outcomes of venous and arterial tissue engineered vascular grafts highlight the importance of coupling long-term implantation studies with computational modeling. Acta Biomater. 2019, 94, 183-194.  
101. Hsu, M. C.; Kamensky, D.; Xu, F.; Kiendl, J.; Wang, C.; Wu, M. C.; Mineroff, J.; Reali, A.; Bazilevs, Y.; Sacks, M. S. Dynamic and fluid-structure interaction simulations of bioprosthetic heart valves using parametric design with T-splines and Fung-type material models. Comput Mech. 2015, 55, 1211-1225.  
102. Martin, C.; Sun, W. Simulation of long-term fatigue damage in bioprosthetic heart valves: effects of leaflet and stent elastic properties. Biomech Model Mechanobiol. 2014, 13, 759-770.  
103. Szafron, J. M.; Ramachandra, A. B.; Breuer, C. K.; Marsden, A. L.; Humphrey, J. D. Optimization of tissue-engineered vascular graft design using computational modeling. Tissue Eng Part C Methods. 2019, 25, 561-570.  
104. Sulejmani, F.; Caballero, A.; Martin, C.; Pham, T.; Sun, W. Evaluation of transcatheter heart valve biomaterials: Computational modeling using bovine and porcine pericardium. J Mech Behav Biomed Mater. 2019, 97, 159-170.  
105. Zakerzadeh, R.; Hsu, M. C.; Sacks, M. S. Computational methods for the aortic heart valve and its replacements. Expert Rev Med Devices. 2017, 14, 849-866.  
106. Abbasi, M.; Barakat, M. S.; Vahidkhah, K.; Azadani, A. N. Characterization of three-dimensional anisotropic heart valve tissue mechanical properties using inverse finite element analysis. J Mech Behav Biomed Mater. 2016, 62, 33-44.  
107. Fernández-Ruiz, I. Computer modelling to personalize bioengineered heart valves. Nat Rev Cardiol. 2018, 15, 440-441.  
108. Butcher, J. T. The root problem of heart valve engineering. Sci Transl Med. 2018, 10, eaat5850.  
109. Simmons, C. A. Taking bioengineered heart valves from faulty to functional. Nature. 2018, 559, 42-43.  
110. Loureiro-Ga, M.; Veiga, C.; Fdez-Manin, G.; Jimenez, V. A.; Calvo-Iglesias, F.; Iñiguez, A. A biomechanical model of the pathological aortic valve: simulation of aortic stenosis. Comput Methods Biomech Biomed Engin. 2020, 23, 303-311.  
111. Conti, C. A.; Della Corte, A.; Votta, E.; Del Viscovo, L.; Bancone, C.; De Santo, L. S.; Redaelli, A. Biomechanical implications of the congenital bicuspid aortic valve: a finite element study of aortic root function from in vivo data. J Thorac Cardiovasc Surg. 2010, 140, 890-896, 896.e1-2.  
112. Arzani, A.; Mofrad, M. R. K. A strain-based finite element model for calcification progression in aortic valves. J Biomech. 2017, 65, 216-220.  
113. Rassoli, A.; Fatouraee, N.; Guidoin, R.; Zhang, Z. Comparison of tensile properties of xenopericardium from three animal species and finite element analysis for bioprosthetic heart valve tissue. Artif Organs. 2020, 44, 278-287.  
114. Vashistha, R.; Kumar, P.; Dangi, A. K.; Sharma, N.; Chhabra, D.; Shukla, P. Quest for cardiovascular interventions: precise modeling and 3D printing of heart valves. J Biol Eng. 2019, 13, 12.  
115. van der Valk, D. C.; van der Ven, C. F. T.; Blaser, M. C.; Grolman, J. M.; Wu, P. J.; Fenton, O. S.; Lee, L. H.; Tibbitt, M. W.; Andresen, J. L.; Wen, J. R.; Ha, A. H.; Buffolo, F.; van Mil, A.; Bouten, C. V. C.; Body, S. C.; Mooney, D. J.; Sluijter, J. P. G.; Aikawa, M.; Hjortnaes, J.; Langer, R.; Aikawa, E. Engineering a 3D-bioprinted model of human heart valve disease using nanoindentation-based biomechanics. Nanomaterials (Basel). 2018, 8, 296.  
116. Labrosse, M. R.; Beller, C. J.; Robicsek, F.; Thubrikar, M. J. Geometric modeling of functional trileaflet aortic valves: development and clinical applications. J Biomech. 2006, 39, 2665-2672.  
117. Marom, G.; Haj-Ali, R.; Rosenfeld, M.; Schäfers, H. J.; Raanani, E. Aortic root numeric model: correlation between intraoperative effective height and diastolic coaptation. J Thorac Cardiovasc Surg. 2013, 145, 303-304.  
118. Zhang, W.; Rossini, G.; Kamensky, D.; Bui-Thanh, T.; Sacks, M. S. Isogeometric finite element-based simulation of the aortic heart valve: Integration of neural network structural material model and structural tensor fiber architecture representations. Int J Numer Method Biomed Eng. 2021, 37, e3438.  
119. Li, Q.; Wang, J.; Tao, H.; Zhou, Q.; Chen, J.; Fu, B.; Qin, W.; Li, D.; Hou, J.; Chen, J.; Zhang, W. H. The prediction model of warfarin individual maintenance dose for patients undergoing heart valve replacement, based on the back propagation neural network. Clin Drug Investig. 2020, 40, 41-53.  
120. Obbink-Huizer, C.; Oomens, C. W.; Loerakker, S.; Foolen, J.; Bouten, C. V.; Baaijens, F. P. Computational model predicts cell orientation in response to a range of mechanical stimuli. Biomech Model Mechanobiol. 2014, 13, 227-236.  
121. Loerakker, S.; Obbink-Huizer, C.; Baaijens, F. P. A physically motivated constitutive model for cell-mediated compaction and collagen remodeling in soft tissues. Biomech Model Mechanobiol. 2014, 13, 985-1001.  
122. Ristori, T.; Bouten, C. V. C.; Baaijens, F. P. T.; Loerakker, S. Predicting and understanding collagen remodeling in human native heart valves during early development. Acta Biomater. 2018, 80, 203-216.  
123. Loerakker, S.; Ristori, T.; Baaijens, F. P. T. A computational analysis of cell-mediated compaction and collagen remodeling in tissue-engineered heart valves. J Mech Behav Biomed Mater. 2016, 58, 173-187.  
124. Soares, J. S.; T, B. L.; Sotiropoulos, F.; M, S. S. Modeling the role of oscillator flow and dynamic mechanical conditioning on dense connective tissue formation in mesenchymal stem cell-derived heart valve tissue engineering. J Med Device. 2013, 7, 0409271-0409272.  
125. Soares, J. S.; Sacks, M. S. A triphasic constrained mixture model of engineered tissue formation under in vitro dynamic mechanical conditioning. Biomech Model Mechanobiol. 2016, 15, 293-316.  
126. Sanders, B.; Loerakker, S.; Fioretta, E. S.; Bax, D. J.; Driessen-Mol, A.; Hoerstrup, S. P.; Baaijens, F. P. Improved geometry of decellularized tissue engineered heart valves to prevent leaflet retraction. Ann Biomed Eng. 2016, 44, 1061-1071.  
127. Loerakker, S.; Argento, G.; Oomens, C. W.; Baaijens, F. P. Effects of valve geometry and tissue anisotropy on the radial stretch and coaptation area of tissue-engineered heart valves. J Biomech. 2013, 46, 1792-1800.  
128. Motta, S. E.; Fioretta, E. S.; Lintas, V.; Dijkman, P. E.; Hilbe, M.; Frese, L.; Cesarovic, N.; Loerakker, S.; Baaijens, F. P. T.; Falk, V.; Hoerstrup, S. P.; Emmert, M. Y. Geometry influences inflammatory host cell response and remodeling in tissue-engineered heart valves in vivo. Sci Rep. 2020, 10, 19882.